This is a synopsis for commercial items, prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. The Acquisition Method being utilized is Full and Open Competition. The National Heart, Lung, and Blood Institute intends to solicit proposals for the purchase of consumables for fluorescent DNA sequencing using ABI PRISM 3730XL DNA Analyzer instruments under solicitation number NHLBI-HG-P-06-049/KLW. The contract is to be awarded as an Indefinite Delivery/Indefinite Quantity Requirements contract for a period of performance of one (1) year with four (4) twelve month renewable option years beginning with the effective date of the contract award. The anticipated effective date of award is February 28, 2006. The consumables to be supplied per year are as follows: 1) #4337458 BigDye Terminator Kits (10 each); 2) #4335611 POP 7 Reagent (30 each); 3) #4316357 POP 6 Reagent (20 each); 4) #4316355 POP 4 Reagent (50 each); 5) #4319899 Array for 3100 (10 each); 6) #402824 Running Buffer for 3100 (90 each). All of these consumables are tailored for use on ABI PRISM 3730XL instruments, in most cases with Applied Biosystems owning the patent rights. These instruments are being used for large-scale sequencing at the NIH Intramural Sequencing Center, Bethesda, MD. Use of the listed consumables must result in the ability to generate high-fidelity sequence data (<1% ambiguous bases over 800 pb reads) using the ABI 3730 instrument. Sequencing reagents must be capable of using cycle-sequencing protocols with high sensitivity and signal-to-noise ratios starting with minimal amounts (e.g. <100 ng) of starting DNA. Consumables must be tailored for use on ABI 3730 instruments for performing DNA sequencing. They must not compromise the warranty or service contract of these instruments. Additionally, the primer cycle sequencing ready reaction kit must be a single energy transfer molecule using a donor fluorescein and an acceptor dichlororhodamine coupled by a highly efficient energy transfer linker to give the brightest signal for the longest read lengths. This is a fixed price requirement. Solicitation No. NHLBI-HG-P-06-049/KLW will be issued as a Request for Proposal (RFP), available in conjunction with this announcement. Any firm believing itself capable of providing these items must respond by submitting a written request to Kesha Williams, Contract Specialist, 6701 Rockledge Dr, Room 6137, Bethesda, MD 20892, [email protected]. The request must include a point of contact name and number, company name, address, telephone number, fax number, and email address; and must be received within fifteen (15) days of publication of this notice. The solicitation will open upon publication of this notice and close on the fifteenth day. All proposal responses will include detailed technical information and other technical literature demonstrating the ability to meet these requirements. A cost proposal detailing all costs associated with this requirement is also necessary. All proposals must be submitted no later than February 14, 2006 at 3:30 P.M. whereupon this solicitation will be closed. The NAICS Code/Size is 325414/500EM. This is a competitive solicitation package. For any questions regarding this announcement, please contact Kesha Williams via email at [email protected]. Point of Contact: The National Heart, Lung and Blood Institute, Office of Acquisitions, Procurement Branch, Rockledge Drive (RKL2/6137) Room 6137, Bethesda, Maryland 20892-7902 Attention: Ms. Kesha Williams, Contract Specialist. The National Heart, Lung and Blood Institute, Office of Acquisitions, Procurement Branch, Rockledge Drive (RKL2/6150) Room 6150, Bethesda, Maryland 20892-7902 Attention: Debra Hawkins, Contracting Officer.

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