Basic Description:
The request is for an automated microplate reader that provides superior flexibility and sensitivity for the definition, finetuning and validation of the wide range of assays and reagents. A reader that supports a broad range of microplate-based detection assays for primary and secondary screening; enzymatic assays including kinase and protease assays; G protein coupled receptor assays; ion channel-related assays; receptor-ligand binding studies and other molecular interaction assays; cell-based assays; DNA/RNA quantification; and applications based on UV fluorometry.


Minimum Essential Characteristics:
1. Premium Quad4 Monochromator technology that reduces stray light down to 2 x 10-7, improves spectral blocking, brings more light onto the sample and the detector, and includes a flash lamp with 2 x more power.

2. A flexible wavelength selection from UV to NIR with high accuracy and adjustable bandwidth settings for fine adjustments towards the fluorophore.

3. High speed recording for excitation, emission, and absorbance spectra in the fast scanning mode.

4. Rapid 3D scanning enabling characterization of unknown samples, and Z-focusing in all top reading modes.

5. A well Scanning mode, heated Incubation from +4C to 42C, and linear or orbital shaking in 3 speeds.

6. Accommodates 6, 12, 24, 48, 96, 384 and 1536 well plate formats. Includes ability to measure wavelength range of in tunable 1nm increments with software-selectable variable bandwidth.

7. Fast ratiometric measurements immediate changes in wavelength in FRET assays and change of polarization direction within one millisecond for FP measurements.

8. On-board control buttons for instant start/stop control of pre-programmed measurement workflows.

9. A luminescence module that provides access to single and dual color luminescence measurements, glow and flash luminescence with three fibers adapted for 96-well, 384-well and 1,536-well plates.

10. Optional injectors with variable syringe size, volume and speed settings.

11. Flexibility
a. Wavelength/Bandwidth/Accuracy (fluorescence intensity)
i. Wavelength selection Premium Quad4 Monochromators
ii. Wavelength range
1. Excitation: 230 nm 850 nm
2. Emission: 280 nm 850 nm
iii. Bandwidth Adjustable
1. less than 300 nm: 2.5 nm 10 nm
2. greater than 300 nm: 5 nm 20 nm
iv. Wavelength accuracy
1. Less than 300 nm: 0.5 nm
2. greater than 300 nm: 1 nm
v. Wavelength precision
1. less than 300 nm: 0.5 nm
2. greater than 300 nm: 1 nm
b. Wavelength/Bandwidth/Accuracy (absorbance)
i. Wavelength selection Double monochromator
ii. Wavelength range 230 nm 1,000 nm
iii. Bandwidth 2.5 nm below 300 nm, 5 nm above
iv. Wavelength accuracy
1. less than 300 nm: 0.5 nm
2. greater than 300 nm: 1 nm
v. Wavelength precision
1. less than 300 nm: 0.5 nm
2. greater than 300 nm: 1 nm
vi. Measurement range 0 4 OD
vii. Linearity 0 3 OD: R2 greater than 0.999 @ 280 nm and 492 nm
viii. Accuracy at 492 nm 1 OD: less than 0.5 % and 0.006 OD
ix. Precision at 492 nm 1 OD: less than 0.5 % and 0.006 OD

12. Sensitivity
a. Fluorescence sensitivity values
i. Fluorescence top reading 384-well plate 0.17 fmol / well
ii. Fluorescence bottom reading 384-well plate 2 fmol / well
iii. TRF 384-well plate 10 amol / well Europium (100 flashes)
iv. FP 384-well plate 2 mP standard deviation @1 nM Fluorescein
b. Luminescence sensitivity values
i. Glow luminescence 384-well plate 0.7 fmol ATP / well
ii. Flash luminescence 384-well plate less than 80 amol ATP / well

Bid Protests Not Available