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NOTICE OF INTENT TO SOLE SOURCE -- The National Institute for Occupational Safety and Health (NIOSH), Toxicology and Molecular Biology Branch (TMBB), HELD in Morgantown, WV has a need for a Guava Personal Cytometer (GPC) to investigate the cellular response to occupational skin exposures, cell number, viability, and induction of apoptosis for all isolated primary cell cultures for projects undertaken by the Morgantown Dermatotoxicology Laboratory under TMBB. We specifically plan to purchase the following items from Guava Technologies, Inc., Burlingame, CA: (1) Cat # 0500-0010, Guava PC which includes Guave Cell Counting and Viability Software module, Protein Expression Analysis Software module, Apoptosis Analysis Software module; (2) Guava ViaCount cell counting and viability reagents; and (3) Guave Check Kit. This unit is able to perform multiple assays: cell counts, cell viability assays and apoptosis assays. The machine requires only 5,000 to 10,000 cells for each analysis, an important feature when there is a limited number of primary cells obtained from a cell isolation. Cell count and viability use a combination of two fluorescent dyes, one which identifies all cells, the other that identifies dead cells. The machine has dedicated software and downloads measurements into an Excel spreadsheet for easy analysis. The equipment does not require special training, thus providing ease of use to other laboratories within the NIOSH, TMBB. There is no other known source available, however, if there are vendors having the capabilities necessary to meet the stated requirements, they are invited to submit appropriate documentation, literature, brochures and references to Brenda L. Goodwin, Reference: 2002-Q-00311, DHHS, PHS, CDC, NIOSH, ALOSH, 1095 Willowdale Road, Morgantown, WV 26505. Telephone inquiries or fax confirmations should be made to 304/285-5882, fax 304/285-6083. Organizations shall respond as soon as possible, but no later than noon local prevailing time on December 28, 2001. Information received will generally be used for the purpose of determining whether to conduct a competitive procurement. This notice is issued for informational purposes only.

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