The Food and Drug Administration is seeking small business sources to provide custom E. coli DNA microarrays. The custom Genechip microarrays described in this solicitation must be compatible to be utilized with an integrated Affymetrix GeneChip Scanner 3000 and Fluidics 450s system.Item (1), microarrays, Qty. 140, EA. Item (2), Shipping, Qty. 140 EA. The arrays must be able to be read/utilized by the Affymetrix GeneChip Scanner 3000 System. The GeneChip Scanner 3000 System is a fully integrated platform for conducting research using the Affymetrix GeneChip? Custom Genotyping assays and GeneChip probe arrays. The arrays must be able to accomodate at least 22,000 unique E. coli genes and 1,000 control sequences (23,000 total probe sets). Furthermore, each of the gene sequence represented on this array must have 11 perfectly matched 25-mer probes as well as 11 mismatch 25-mer probes in order to estimate nonspecific hybridization. This results in an array capable of accomodating greater than 500,000 individual features. Additionally, the size of each of these features must be 11 microns. The arrays must contain the following hybridization controls: bioB, bioC, bioD and cre.The arrays must contain a normalization control set of 100 probe sets. REQUEST FOR QUOTE (RFQ) any firm that believes it is capable of providing the required service as stated herein may submit a quote which will be considered if received by the fifteenth (15th) calendar day following the appearance of this announcement. A determination to compete this procurement based on a response to this notice is solely within the discretion of the Government. All responses or questions regarding this posting must be in writing and can be sent via email to [email protected]. No phone calls will be accepted. An award will be made in fifteen (15) calendar days from the date of this notice. This procurement will be awarded as a simplified acquisition for commercial items in accordance with FAR Subpart 12.6, as supplemented with additional information included in this notice.

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