Service Area Office (SAO) - East intends to negotiate on a sole source basis Nikon Instruments, Inc, 1300 Walt Whitman Road, Melville, NY, 11747-3064, to purchase a Nikon High Content/Confocal/SIM/ STORM system for VA Puget Sound Health Care System - Seattle Division. The investigators of the Cellular and Molecular Imaging Core require equipment with a comprehensive range of state-of-the-art imaging modalities that must integrate with the microscopes currently housed in the Cellular and Molecular Imaging Core laboratory. The laboratory already houses three Nikon microscope systems (90i, TiE and NiE) with workstations running proprietary NIS Elements software, only available from Nikon. The following equipment will be purchased: ITEM INFORMATION ITEM NUMBER DESCRIPTION OF SUPPLIES/SERVICES QUANTITY UNIT 0001 Nikon High Speed, Widefield Imaging System 1 - MEA54000 Ti2-E Inverted Motorized Microscope Stand, Motorized Light Distribution; Eye 100/Left 100/Right 100/Left 80/ Eye Prisms, Side port FOV 22mm C-Mount or 25mm F-Mount, Manual Switching Intermediate Magnification Lens with Status Detection 1.0-1.5x (1.5x exchangeable to 2.0x), Manual In/Out and Manual Focus Bertrand Lens with Status Detection, Built-In Hub for Motorized Accessory Connection, Includes 2 ISO C-Mount Side port Adapters, Main Cable, Cable Clips and Toolkit 1 - MEF55037 Ti2-E Controller, Provides Power for Motorized Stand and Motorized Components, USB and LAN Connection, Two Connectors Accommodating Four I/O Channels Each 1 - MEB55830 Ti2-T-BS Binocular Body Base Unit (Simple) 1 - MEB52340 TC-T-TS S Binocular Tube S 2 - MAK10110 CFI 10X EYEPIECE F.N. 22MM-NC 1 - MEP59394 Ti2-ND-P Perfect Focus System 4, 340nm-800nm, Six Place DIC Motorized Nosepiece with Motorized Reflector, Measure Unit, 45mm IR BlockFilter for Diascopic Illumination, Pinch Guard <(>&<)> Drip Hose 1 - MEC56120 TI2-S-SE-E Motorized Stage with Encoder, XY Stroke: 114mm x 75mm, Repeat Position Accuracy +/- 0.5 m (or less) 1 - MEF55705 TI2-S-JS Joystick, XY Control of Motorized Stage, Z-Focus Control of Microscope, PFS On/Off, Programable Function Buttons for Microscope component Control, Liquid Crystal Display that Indicates Status of Microscope 1 - MEC59140 TI2-S-HU Universal Adjustable Holder for Motorized Stage, Holds Petri Dishes and Slides 1 - MEC59150 TI2-S-HW Well Plate Holder for Motorized Stage 1 - 91989 Ti/Ti2 Piezo Stage Insert with USB Controller 1 - 91995 Petri Dish Holder 35MM for Piezo Stage 1 - 91997 Universal Holder for Piezo Stage 1 - 91994 Multi-Well Plate Holder for Piezo Stage 1 - 91998 Multiple Slide Holder Insert for Piezo Stage 1 - MQS41320 NIS-Elements: Hardware Module: Motorized Focus Control - device control for external motorized focus control. Includes Prior, Maerzhauser, Nikon RFA, and PI Piezo Z drive (note that piezo is via RS232 only). 1 - MRL00022 CFI60 Plan Achromat Ultra-Wide 2x Objective Lens, N.A. 0.06, W.D. 7.5mm, F.O.V 25mm 1 - MRD00045 CFI60 Plan Apochromat Lambda 4x Objective Lens, N.A. 0.2, W.D. 20.0mm, F.O.V. 25mm 1 - MRD00105 CFI60 Plan Apochromat Lambda 10x Objective Lens, N.A. 0.45, W.D. 4.0mm, F.O.V. 25mm, DIC, Spring Loaded 1 - MRD00205 CFI60 Plan Apochromat Lambda 20x Objective Lens, N.A. 0.75, W.D. 1.0mm, F.O.V. 25mm, DIC, Spring Loaded 1 - MRD00405 CFI60 Plan Apochromat Lambda 40x Objective Lens, N.A. 0.95, W.D. 0.17-0.25mm, F.O.V. 25mm, DIC, Correction Collar 0.11-0.23mm, Spring Loaded 1 - MEL51920 Ti2-C-TC-E Motorized Condenser Turret, Seven Position, Aperture Diaphragm, ND32 Filter In Holder, Light Blocking Module, Labels 1 - MEL56200 T-C-LWD Lens Unit for System Condenser Turret, N.A. 0.52, W.D. 30mm 1 - MEE54860 TI2-LA-BF Fixed Main Branch 1 - MEE54730 TI2-LA-FLL Epi Fluorescence Module Large Field of View, Fiber Input 1 - MEV51030 Ti2-F-FLT-E Motorized Epi Fluorescence Turret, Six Position, Compatible with 25mm and 32mm Fluor Cubes 1 - MXA22147 S-TI2-DCL Daisy Cable Long, Connects Filter Turrets and Manual L-Apps Main Branches to Hub for Intelligent Mirror Position 1 - 77060078 AURA II Solid State Triggerable Illuminator: 4 channel light source (395nm, 485nm, 560nm, 640nm); Note: LLG must be purchased separately. 1 - 77060101 Liquid Light Guide for Lumencor; 3mm diameter, 2m, VIS, with protective metal outer jacket 1 - 77074658 DAPI/FITC/CY3/CY5 Emission Filter Set for Spectra X for use with77074657.Emitter: ET435/26m, 32mmEmitter: ET515/30m, 32mm Emitter: ET595/40m, 32mmEmitter: ET705/72m, 32mm 1 - 96369 C-FLL Large Field of View DAPI SOLA Filter Set, High-Signal-Noise, Semrock BrightLine® 1 - 96372 C-FLL Large Field of View GFP/FITC/Cy2 Filter Set, High-Signal-Noise, Semrock Brightline® 1 - 96375 C-FLL Large Field of View Texas Red/mCherry Filter Set, High-Signal-Noise, Semrock Brightline® 1 - 96376 C-FLL Large Field of View Cy5 Filter Set, High-Signal-Noise, Semrock Brightline® 1 - MQS41930 Device control for of Sutter DG4/5, AOTF, Agilent, Lumencor SpectraX, Spinning Disk, etc. (Does NOT Includes the Wavelength Control Module (MQS41220) for filter control) 1 - 77023027 NI-BB Triggered device hub for direct hardware triggering of optomechanical devices. Includes National Instruments 1- PCIe 6323 NIDAQ card and trigger cable set. Requires appropriate hardware drivers and triggered device control modules for NIS-Elements 1 - MQS41920 Controls Shutter and Illumination Intensity from EXFO and SOLA (RS232slow Control) Light Sources Note: Control for Sutter Lambda XL, but MQS41220 also needed 1 - 77018292 Photometrics Prime 95B-25MM Back-illuminated sCMOS Camera. 1608x1608,30fps@16-bits, 11um pixel. -10C air or -25C water cooling, PCIe interface, F-mount connection. ~95% peak QE. 1 - MQS42780 Device Control for Externally Triggering a Device Through a Camera *MQS41320 is a Required Module for Triggered Piezo Z *MQS41930 is a Required Module for Triggered DG4/5 Available for C, HC, AR & BR only 1 - 77062007 HS-632 TTL-triggered high speed filter wheel. F-mount camera port, 6positions for 32mm emission filters. Includes Ti2 side port mount 1 - 77074655 DAPI/FITC/CY3/CY5 Polychroic and Quad Bandpass Emitter Set For SpectraX mounted in Ti2 Cube Dichroic: T395/470/550/640pc, 32x44x2mmEmitter: ET430/36;513/44;595/41;719/105m, 32mm 1 - MQS41220 NIS-Elements: Hardware Module: Wavelength control - device control for Sutter, Prior and Unibliz illumination devices including shutters and filter wheels 1 - MQS43040 NIS-Elements High Content Upgrade Module- adds high content well plate acquisition, database, analysis and review to NIS-Elements AR, AR Passive and C packages. MQS42650 6D Imaging needed for AR, AR ML. MQS42560 6D. Imaging not needed for AR Passive MQS31500. 1 - 77049612 Series 320 Vibration Isolation Platform with 4 VIB320 bearings, 24 x 30 plate capable of a payload between 65 and 120 pounds. Price does not include Freight. 1 - 92084 Large Nylon Dust Cover, 14" Wide x 26 5" Deep x 32" High 3 - 97050 USB 2.0 A-B 15ft Cable 10 - 79035 Power Cord 120V, Lead Free 1 - MXA22158 S-Ti2I-LU Interlock Cable between Ti2-E, Ti2-A and LU-4A 1 - MXA22168 30cc Non-Fluorescing Immersion Oil F 1.00 JB 0002 Nikon A1R-nSTORM Confocal and Localization Super Resolution System 1 - 999-30 Nikon A1R-STORM System Complete This system is built on the incredibly stable Ti2 platform with motorized XYZ, and includes Nikon A1r Live Cell Resonant Dual Scanner, A1-DUG Hybrid GaAsP/PMT 4ch Detector System, Transmitted Light Detector , Nikon LUN4 4 Line Solid State Laser System, HP LUNF 100mW per line at 405, 488, and 561nm and 125mW at 640nm, motorized TIRF illumination, Super Resolution 100x/1.49 objective, Nikon Perfect Focus System, LED Based Epi Fluorescent System (DAPI, FITC, TRITC cubes), LED Based Transmitted Light System , 10x, 20x, 40x oil, 60x oil Objectives, DIC Imaging, NIS-Elements software for control and N-STORM analysis, HP Workstation with High End GPU, 30 inch High Resolution Monitor, and Anti-Vibration Table 30x48 Inch. Package Also Includes Nikon System Optimal Performance Package for STORM and A1R. 1.00 JB 0003 Nikon nSIMe Structured Illumination Super Resolution System 1 - Nikon SIMe System Complete. This system is built on the incredibly stable Ti2 platform with motorized XYZ, and includes LUN3-SIM 488, 561, 640 Laser for SIMe, Motorized SIM Illuminator, SIM filter turret with Quad-band Dichroic, Nikon Perfect Focus System, LED Based Epi Fluorescent System (DAPI, FITC, TRITC cubes), LED Based Transmitted Light System, 10x, 20x, 100x SR oil Objective, SR Silicon Objective, Nikon NIS-Elements HCA Software Package, HP Z840 Workstation, 30 inch High Resolution Monitor, and Anti-Vibration Table 48x72 Inch. Package Also Includes Nikon System Optimal Performance Package for SIMe. 1.00 JB 0004 NIKON Excitation/Emission Splitting Cubes 3 - 96360 C-FL DAPI Hard Coat, High Signal-to-Noise, Zero Shift Filter Set,Excitation: 350/50nm (325-375nm), Emission: 460/50nm (435-485nm), Dichroic Mirror: 400nm 3 - 96362 C-FL GFP Hard Coat, High Signal-to-Noise, Zero Shift Filter Set, Excitation: 470/40nm (450-490nm), Emission: 525/50nm (500-550nm), Dichroic Mirror: 495nm 1 - 96228 CF-L AT TRITC/Cy3 Long Pass Filter Set, Excitation: 540/25nm (527.5-552.5nm), Emission: 575 Long Pass, Dichroic Mirror: 565nm 3 - 96365 C-FL Texas Red Hard Coat, High Signal-to-Noise, Zero Shift Filter set, Excitation: 560/40nm (540-580nm), Emission: 630/70nm (593-668nm), Dichroic Mirror: 585nm 2 - 96366 C-FL CY5 Hard Coat, High Signal-to-Noise, Zero Shift Filter Set, Excitation: 620/60nm (590-650nm), Emission: 700/75nm (663-738nm), Dichroic Mirror: 660nm 1.00 JB 0005 NIKON CFI60 Objectives 3 - MRD70200 CFI60 Plan Apochromat VC 20x Objective Lens, N.A. 0.75, W.D. 1.0mm, F.O.V. 25mm, DIC, Spring Loaded 1 - MRD00045 CFI60 Plan Apochromat Lambda 4x Objective Lens, N.A. 0.2, W.D. 20.0mm, F.O.V. 25mm 1 - MRD00105 CFI60 Plan Apochromat Lambda 10x Objective Lens, N.A. 0.45, W.D. 4.0mm, F.O.V. 25mm, DIC, Spring Loaded 1 - MRD00405 CFI60 Plan Apochromat Lambda 40x Objective Lens, N.A. 0.95, W.D. 0.17-0.25mm, F.O.V. 25mm, DIC, Correction Collar 0.11-0.23mm, Spring Loaded 1.00 JB 0006 NIS Elements Control and Analysis software 2 - MQS31410 NIS-Elements: Advanced Research Software Upgrade Agreement (AR-SUA) 2 - MQS43130 NIS-ELEMENTS JOBS ACQUISITION MODULE FOR CUSTOM ROUTINES. AVAILABLE FORC AND AR ONLY. MQS42560 6D IMAGING REQUIRED. 2 - 77010604 Image processing and measurement analysis engine for custom and standard routines with support of ND dimensions, 3D features, Javascript support, output controls and many data management functions. This module includes both originally released General Analysis (GA2) (MQS43110) and GA3. (Includes MQS43110 and MQS43150) Available for C, HC, AR or AR Passive Only 1 77010603 Adds GA3 capabilities to licenses with existing MQS43110 (original General Analysis GA2): Adds Image processing and measurement analysis engine for custom and standard routines with support of ND dimensions,3D features, Javascript support, output controls and many data management functions. Available for C, HC, AR or AR Passive Only 1.00 JB 0007 Scientific Imaging Cameras 1 - MQA17500 DS-Qi2 monochrome CMOS camera 1 - MQA17000 DS-Ri2 Color CMOS camera 2 - MQF52056 AC Adapter 2 - 79035 Power Cord 120V, Lead Free 2 - USB3 Cable, USB3.0 A to MicroB plug (angled) with locking screws, 3m. 2 - MED53140 TI-BDTV2 F-TV TUBE F-MOUNT 2 - MQD43000 DS-F F-MOUNT ADAPTER 1X 1 - MQA11550 DS-Qi1 Quantitative Monochrome Camera Head. 1280 x 1024 pixels, 6.45 um pixel DISCONTINUED 1 - MQA16050 DS-Ri1 Cooled Digital Camera Head, 1280 x 1024 pixels, up to 12.7MP using pixel shift, 6.45 um pixel, (DISCONTINUED Replaced by DS-Ri2) 1 - MQD42005 C-DA C-Mount/Iso Camera Adapter 1x 1.00 JB This notice of intent is not a request for competitive proposals. Any responsible sources who wish to identify their interest and capability to provide this equipment or equipment that is compatible must notify this Contracting Specialist at [email protected] NLT Monday, November 26, 2018 by 4:00 PM EST. Telephone responses will not be accepted. Upon receipt of the interested parties' notification, the Contracting Officer will determine whether to conduct a competitive procurement or proceed with sole source negotiations.

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