The PCO-EAST (Program Contracting Office) East intends to negotiate on a sole source basis with Nikon Instruments, Inc., 1300 Walt Whitman Road, Melville, NY, 11747, to purchase an upgrade of the Ralph H. Johnson VAMC's specialized microscope package, built on the Nikon TiU platform, which is fully compatible with the already-existing core system software package and data analysis tools. It is imperative to purchase the same equipment to maintain consistency and continuity of research in progress. The following equipment will be purchased: ITEM NUMBER DESCRIPTION OF SUPPLIES/SERVICES QUANTITY UNIT Nikon TiU Microscope 1 Inverted Microscope Eclipse Ti-U Main Body equipped with: left and rightside ports, intermediate magnification changer 1.5X, coaxial coarse/fine focus w/tension adjustment on coarse and focus stop on coarse, laser safety interlock signal output; Light-distribution: 100 Eyepiece, 100Left, 100 Right (*), and Auxiliary position (*) Note: (*) prism can be exchanged for optional prisms; Eye20/Left80, Eye20/Right80 1.00 EA 2 Ti-T-B Eyepiece Base Unit 1.00 EA 3 Ti-TS Eyepiece Tube S; Siedentopf type, 25 degrees inclination angle eye level, Interpupillary distance 51-80mm; Erect image, locking ring forone hand diopter adjustment 1.00 EA 4 CFI 10X EYEPIECE F.N. 22MM-NC 2.00 EA 5 Ti-ND6 Sextuple DIC Nosepiece 1.00 EA 6 Ti-DH Dia Pillar Illuminator 100W 1.00 EA 7 Transmitted white light LED light source with flip in filter for Tiseries microscope: a light source with integrated power supply andhousing (no microscope adapter is required for Ti series), with a remote rheostat dial. Includes BNC connection for TTL control (compatible with NIDAQ triggered control). Optional cable for non-triggered control is available 1.00 EA 8 USB to TTL cable to control TTL-driven shutters and light sources (not for use with triggered control) 1.00 EA 9 Filter 45mm, ND8A multi-coated neutral density 1.00 EA 10 Filter 45mm, ND16A multi-coated neutral density 1.00 EA 11 Large Nylon Cover, optional 14" wide x 26.5" deep x 32" high 1.00 EA 12 Immersion Oil Type B; 1 Ounce 1.00 EA DIC/Phase Components 13 Ti-C System Condenser Turret 1.00 EA 14 Ti-C-LWD LWD Lens Unit for System Condenser Turret 1.00 EA 15 PH-1 Module for LWD, TE-C (10x, 20x), 1.00 EA 16 T-P2 DIC Polarizer 1.00 EA 17 1 Ti-A Analyzer Block 1.00 EA 18 T-C DIC Module LWD N1 Dry 1.00 EA 19 T-C DIC Module LWD N2 Dry 1.00 EA 20 D-C DIC Slider 10X 1.00 EA 21 D-C DIC Slider 40X II 1.00 EA 22 D-C DIC Slider 60X II 1.00 EA Optics 23 "CFI60 Plan Fluor 4x Objective Lens, N.A. 0.13, W.D. 17.2mm, F.O.V. 25mm" 1.00 EA 24 "CFI60 Achromat Phase Contrast ADL LWD 20x Objective Lens, N.A. 0.4, W.D 3.1mm, 1.00 EA 25 F.O.V 22mm, Ph1, Fixed Correction 1.2mm" 1.00 EA 26 "CFI60 Plan Fluor 10x Objective Lens, N.A. 0.3, W.D. 16mm, F.O.V. 25mm, DIC" 1.00 EA 27 CFI60 Plan Fluor 40x Oil Immersion Objective Lens, N.A. 1.3, W.D. 0.2mm, F.O.V.25mm, DIC 1.00 EA 28 "CFI60 Plan Apochromat Lambda 60x Oil Immersion Objective Lens, N.A. 1.4, W.D. 0.13mm, F.O.V. 25mm, DIC" 1.00 EA Manual Stage 29 TI-SR Rectangular Stage; 70mm x 50mm travel; Includes two circular stageinserts; 25mm opening (with cutout for oiling objective),and 40mm opening 1.00 EA 30 60MM Petri Dish holder and 1 x 3 Slide Holder - Stage Insert Ring 1.00 EA Fluorescence with Solid State LED Iluminator 31 TI-FLC Epi-FL Filter Turret Six position turret, ergonomicallypositioned light shut-off shutter, "Noise Terminator" included for highsignal-to-noise fluorescence images 1.00 EA 32 TI-FL Epi-FL Attachment Wide Field Fluorescence Illuminator: Right angle illuminator with spring loaded adjustable support leg Removeable and Centerable Field Diaphragm, Centerable Aperture Diaphragm, ND4 and ND8 filter sliders, Built-in heat filter, light-shielding plate, Lamp centering target (objective mount) Four bolt mounting system for improved stability. Improved chromatic correction 405-633nm, Includes HMX adapter. 1.00 EA 33 X-Cite 120LED System. Includes: LED Head, PowerCUBE Controller, SpeedDIAL Remote, Microscope Coupling Flange for Nikon Scope (Ti/Ni),manual disk, quick start guide, hex key, USB cable, and power cord. 1.00 EA 34 C-FL DAPI HC Sola HISN Zero Shift Filter Set 1.00 EA 35 C-FL GFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 470/40nm (450-490nm), Emission: 525/50nm (500-550nm), Dichroic Mirror: 495nm 1.00 EA 36 C-FL DS Red, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 545/30nm (530-560nm), Emission: 620/60nm (590-630nm), Dichroic Mirror: 570nm 1.00 EA 37 C-FL CY5, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 620/60nm (590-650nm), Emission: 700/75nm (663-738nm), Dichroic Mirror: 660nm 1.00 EA Nikon DS Qi2/Ri2 CMOS 16MP cameras 38 DS-Ri2 Color CMOS camera 1.00 EA 39 USB3 Cable, USB3.0 A to MicroB plug (angled) with locking screws, 3m. 1.00 EA 40 DS-Qi2 monochrome CMOS camera 1.00 EA 41 AC Adapter 2.00 EA 42 "Power Cord 120V, Lead Free " 2.00 EA 43 USB3 Cable, USB3.0 A to MicroB plug (angled) with locking screws, 3m. 2.00 EA 44 TI-BDTV2 F-TV TUBE F-MOUNT 2.00 EA DS-F F-MOUNT ADAPTER 1X 2.00 EA NIS Elements BR and Control System 45 NIS-Elements: Basic Research: Acquisition and analysis package, includes camera and Nikon motorized microscope drivers. Options modules include, advanced interpreter, live compare, time measurements, image database and 4D acquisition. Note separate drivers are required for device control. 1.00 EA 46 NIS-Elements: Module: 4D imaging - acquisition module for up to 4 dimensions, including any two of the following, time, channel or wavelength and focus. Note that device drivers are required. Available for Basic and Advanced Research only. 1.00 EA 47 Controls shutter and illumination intensity from EXFO and SOLA (RS232slow control) light sources. Adds control for Sutter Lambda XL, but MQS41220 also needed 1.00 EA 48 "NIS-Elements: Hardware Module: Wavelength control - device control for Sutter, Prior and Unibliz illumination devices including shutters and filter wheels." 1.00 EA 49 64bit Imaging workstation with Xeon 3.7GHz 4 Core CPU, 32GB DDR4 RAM,4TB (2x2TB striped RAID) Hard drive, 2GB Video, optical DVDRW, x2 LANethernet adapters, x8 USB 3 and x2 USB 2 ports, keyboard and laseroptical mouse, and recovery software included. Windows 7 64bitprofessional edition. Includes 3 years warranty for parts, labor, and service. 1.00 EA 50 24" LED Backlit IPS monitor with height, swivel, and tilt adjustment. Connects to PC by VGA, Display Port, DVI-D. 178° viewing angle,1920x1200. Includes 3 years parts, labor, and on-site service warranty. 1.00 EA ITEM NUMBER DESCRIPTION OF SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT Nikon TiU Microscope 1 Inverted Microscope Eclipse Ti-U Main Body equipped with: left and rightside ports, intermediate magnification changer 1.5X, coaxial coarse/fine focus w/tension adjustment on coarse and focus stop on coarse, laser safety interlock signal output; Light-distribution: 100 Eyepiece, 100Left, 100 Right (*), and Auxiliary position (*) Note: (*) prism can be exchanged for optional prisms; Eye20/Left80, Eye20/Right80 1.00 EA 2 Ti-T-B Eyepiece Base Unit 1.00 EA 3 Ti-TS Eyepiece Tube S; Siedentopf type, 25 degrees inclination angle eye level, Interpupillary distance 51-80mm; Erect image, locking ring forone hand diopter adjustment 1.00 EA 4 CFI 10X EYEPIECE F.N. 22MM-NC 2.00 EA 5 Ti-ND6 Sextuple DIC Nosepiece 1.00 EA 6 Ti-DH Dia Pillar Illuminator 100W 1.00 EA 7 Transmitted white light LED light source with flip in filter for Tiseries microscope: a light source with integrated power supply andhousing (no microscope adapter is required for Ti series), with a remote rheostat dial. Includes BNC connection for TTL control (compatible with NIDAQ triggered control). Optional cable for non-triggered control is available 1.00 EA 8 USB to TTL cable to control TTL-driven shutters and light sources (not for use with triggered control) 1.00 EA 9 Filter 45mm, ND8A multi-coated neutral density 1.00 EA 10 Filter 45mm, ND16A multi-coated neutral density 1.00 EA 11 Large Nylon Cover, optional 14" wide x 26.5" deep x 32" high 1.00 EA 12 Immersion Oil Type B; 1 Ounce 1.00 EA DIC/Phase Components 13 Ti-C System Condenser Turret 1.00 EA 14 Ti-C-LWD LWD Lens Unit for System Condenser Turret 1.00 EA 15 PH-1 Module for LWD, TE-C (10x, 20x), 1.00 EA 16 T-P2 DIC Polarizer 1.00 EA 17 1 Ti-A Analyzer Block 1.00 EA 18 T-C DIC Module LWD N1 Dry 1.00 EA 19 T-C DIC Module LWD N2 Dry 1.00 EA 20 D-C DIC Slider 10X 1.00 EA 21 D-C DIC Slider 40X II 1.00 EA 22 D-C DIC Slider 60X II 1.00 EA Optics 23 "CFI60 Plan Fluor 4x Objective Lens, N.A. 0.13, W.D. 17.2mm, F.O.V. 25mm" 1.00 EA 24 "CFI60 Achromat Phase Contrast ADL LWD 20x Objective Lens, N.A. 0.4, W.D 3.1mm, 1.00 EA 25 F.O.V 22mm, Ph1, Fixed Correction 1.2mm" 1.00 EA 26 "CFI60 Plan Fluor 10x Objective Lens, N.A. 0.3, W.D. 16mm, F.O.V. 25mm, DIC" 1.00 EA 27 CFI60 Plan Fluor 40x Oil Immersion Objective Lens, N.A. 1.3, W.D. 0.2mm, F.O.V.25mm, DIC 1.00 EA 28 "CFI60 Plan Apochromat Lambda 60x Oil Immersion Objective Lens, N.A. 1.4, W.D. 0.13mm, F.O.V. 25mm, DIC" 1.00 EA Manual Stage 29 TI-SR Rectangular Stage; 70mm x 50mm travel; Includes two circular stageinserts; 25mm opening (with cutout for oiling objective),and 40mm opening 1.00 EA 30 60MM Petri Dish holder and 1 x 3 Slide Holder - Stage Insert Ring 1.00 EA Fluorescence with Solid State LED Iluminator 31 TI-FLC Epi-FL Filter Turret Six position turret, ergonomicallypositioned light shut-off shutter, "Noise Terminator" included for highsignal-to-noise fluorescence images 1.00 EA 32 TI-FL Epi-FL Attachment Wide Field Fluorescence Illuminator: Right angle illuminator with spring loaded adjustable support leg Removeable and Centerable Field Diaphragm, Centerable Aperture Diaphragm, ND4 and ND8 filter sliders, Built-in heat filter, light-shielding plate, Lamp centering target (objective mount) Four bolt mounting system for improved stability. Improved chromatic correction 405-633nm, Includes HMX adapter. 1.00 EA 33 X-Cite 120LED System. Includes: LED Head, PowerCUBE Controller, SpeedDIAL Remote, Microscope Coupling Flange for Nikon Scope (Ti/Ni),manual disk, quick start guide, hex key, USB cable, and power cord. 1.00 EA 34 C-FL DAPI HC Sola HISN Zero Shift Filter Set 1.00 EA 35 C-FL GFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 470/40nm (450-490nm), Emission: 525/50nm (500-550nm), Dichroic Mirror: 495nm 1.00 EA 36 C-FL DS Red, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 545/30nm (530-560nm), Emission: 620/60nm (590-630nm), Dichroic Mirror: 570nm 1.00 EA 37 C-FL CY5, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 620/60nm (590-650nm), Emission: 700/75nm (663-738nm), Dichroic Mirror: 660nm 1.00 EA Nikon DS Qi2/Ri2 CMOS 16MP cameras 38 DS-Ri2 Color CMOS camera 1.00 EA 39 USB3 Cable, USB3.0 A to MicroB plug (angled) with locking screws, 3m. 1.00 EA 40 DS-Qi2 monochrome CMOS camera 1.00 EA 41 AC Adapter 2.00 EA 42 "Power Cord 120V, Lead Free " 2.00 EA 43 USB3 Cable, USB3.0 A to MicroB plug (angled) with locking screws, 3m. 2.00 EA 44 TI-BDTV2 F-TV TUBE F-MOUNT 2.00 EA DS-F F-MOUNT ADAPTER 1X 2.00 EA NIS Elements BR and Control System 45 NIS-Elements: Basic Research: Acquisition and analysis package, includes camera and Nikon motorized microscope drivers. Options modules include, advanced interpreter, live compare, time measurements, image database and 4D acquisition. Note separate drivers are required for device control. 1.00 EA 46 NIS-Elements: Module: 4D imaging - acquisition module for up to 4 dimensions, including any two of the following, time, channel or wavelength and focus. Note that device drivers are required. Available for Basic and Advanced Research only. 1.00 EA 47 Controls shutter and illumination intensity from EXFO and SOLA (RS232slow control) light sources. Adds control for Sutter Lambda XL, but MQS41220 also needed 1.00 EA 48 "NIS-Elements: Hardware Module: Wavelength control - device control for Sutter, Prior and Unibliz illumination devices including shutters and filter wheels." 1.00 EA 49 64bit Imaging workstation with Xeon 3.7GHz 4 Core CPU, 32GB DDR4 RAM,4TB (2x2TB striped RAID) Hard drive, 2GB Video, optical DVDRW, x2 LANethernet adapters, x8 USB 3 and x2 USB 2 ports, keyboard and laseroptical mouse, and recovery software included. Windows 7 64bitprofessional edition. Includes 3 years warranty for parts, labor, and service. 1.00 EA 50 24" LED Backlit IPS monitor with height, swivel, and tilt adjustment. Connects to PC by VGA, Display Port, DVI-D. 178° viewing angle,1920x1200. Includes 3 years parts, labor, and on-site service warranty. 1.00 EA GRAND TOTAL This notice of intent is not a request for competitive proposals. Any responsible sources who wish to identify their interest and capability to provide this equipment must notify this Contracting Officer at [email protected] NLT Friday, April 1, 2016 by 5:00 PM EST. Telephone responses will not be accepted. Upon receipt of the interested parties' notification, the Contracting Officer will determine whether to conduct a competitive procurement or proceed with sole source negotiations.

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