The National Institute of Child Health and Human Development is seeking sources to provide laboratory analysis of 6500 serum and 6500 urine specimens obtained from pregnant women during a previous Study. Analysis of serum specimens will be for soluble fms-like tyrosine kinase 1 (sFlt1 or sVEGFR1), free placental growth factor (PGF or PlGF), and free vascular endothelial growth factor A (VEGF), using enzyme-linked immunoassay (ELISA) kits obtained from R&D Systems Inc. (Minneapolis, MN in order to be consistent with other analysis undertaken by NICHD) as directed by NICHD. VEGF analyses must use an assay as sensitive as possible in order to be able to provide useful measurements of serum concentrations as low as 1 pg/ml or lower. Analysis of urine specimens will be for free PGF and for creatinine. Results will be entered on an Excel spreadsheet provided by NICHD, or as otherwise directed by NICHD. Requirements include significant experience performing ELISAs involving thousands of specimens; in depth knowledge of the particular ELISAs to be used; experience in conducting high sensitivity ELISAs for VEGF, capable of detecting 1 pg/ml or less; ability to confirm the validity of the urinary PGF ELISA; and the presence of experienced, skilled technical support to troubleshoot and monitor the performance of the ELISAs. All ELISAs to be performed by no more than two different laboratory technicians in order to maintain consistency of the laboratory results. This is not a request for proposals. Any Small Businesses and/or 8(a) organizations who feels they have these capabilities should submit a brief response (no more than 10 pages) which indicates their capabilities, the size of their business, (if they are eligible under 8(a) or other set-asides), and information on the management structure of the organization. They should also indicate whether and in what discipline they may have PhD staff that could provide relevant expertise to this project. Resumes of all personnel listed in your response should be provided as an attachment. All responses (an original and 2 copies) must be mailed on or before June 29, 2004, to: Mr. Harvey Shifrin, Contracting Officer, NIH, NICHD, CMB, 6100 Executive Bldg., Room 7A07, 9000 Rockville Pike, MSC 7510, Bethesda, Maryland 20892-7510 (if sending by overnight carrier, 6100 Executive Blvd., Rockville, Maryland 20852). Questions may be e-mailed to [email protected] or phoned to 301-496-4611.

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