Specifically, NIAID, VRC, FCC is seeking one (1) BD FAC Symphony S6 Cell Sorter system (Brand Name or Equal) that is capable of providing the minimum technical requirements listed below:

• Capacity to accommodate up to 50 parameters for deep immunophenotyping.
• 4 nozzles ranging in size from 70um to 130um for large immune cells often sorted in the high containment lab.
• 6-way sorting capability into multiple collection device configurations including microscope slides and plates.
• Temperature control option.
• Bubble detector to detect air in the sample line.
• Automated clog detection and sort protection system.
• Optical sensitivity and resolution to detect bacteria.
• Is functionally compatible with the Baker BioProtect III Biosafety cabinet that the unit will be operated within.
• Allows continuation of ongoing experiments.
• Must have five (5) lasers in the standard configuration, potentially adding 4 lasers.
• Must contain simultaneous 50 parameter detection of 48 fluorescence markers plus forward scatter (FSC) and side scatter (SSC) signals. Upgradable to at least 60 parameters with increased laser capability.
• Must include LED pulser technology or equivalent measurements for Quality Control (QC) by measuring relative background.
• Must use high quality square form PMT and GaAs/GaAsP detectors.
• Requires the use of standard FACS-DIVA software, which is compatible with existing instruments, including the other existing BD-S6 cell sorter.
• Must process Flow Cytometry Standard files in version.3.1 for use in the BD-FlowJo software for standard and advanced multidimensional data analysis.
• Must include a twelve (12) month warranty that will begin upon installation.
• Services to include on-site labor and install and travel during normal business hours.