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The specialized reagents are required to allow our staff at the NICHD to isolate RNA from single cells and to generate cDNA libraries from this RNA. Most importantly, these reagents will allow our staff to tag each RNA so that it is only counted once in our downstream analyses. These processes required highly specialized reagents and highly specialized and expensive equipment. Specifically, our staff need to use the 10xGenomics Microfluids Platform already available on campus. This machine requires 10xGenomics reagents enough for 20 reactions. This is because our staff need to generate enough statistical power to answer the question of whether RNAs from genes expressed at low levels are expressed from one parental allele or from both in each cell.
In summary, our staff must use single cell RNA sequencing with uniquely tagged RNAs for our study. Based on our consultations with experts, we agree that the 10xGenomics Microfluids Platform is the best system for our study and moreover it is the only system available to us. Only 10xGenomics reagents work on this Platform.
The 10xGenomics Chromium system will enable us to discover otherwise inaccessible genomic information and specifically will allow us to determine the parental origin of each RNA species from a single cell. Proprietary microfluid circuits rapidly partition single cell input samples, which are then barcoded using GemCode proprietary technology. The system has a library of up to 4,000,000 available barcodes which is necessary for us to analyze 1000's of RNAs from 1000's of cells. As described above, we will use a Platform already available on campus. We need to purchase the reagents to generate the single cell cDNA libraries that will be compatible with this platform.
10X Genomics, Inc. is the exclusive manufacturer of and the sole supplier authorized to promote the product code 120237.
The proposed contract action is for supplies or services for which the Government intends to solicit and negotiate with only one source under authority of the 41 U.S.C. 253(c) (1), FAR 6.302.
The offeror must include a completed copy of the provision of FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items with its offer. The provisions of FAR Clause 52.212-1 Instructions to Offerors - Commercial Items; FAR Clause 52.212-2, Evaluation - Commercial Items; FAR Clause 52.212-4, Contract Terms and Conditions - Commercial Items; and FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items - Deviation for Simplified Acquisitions applies to this acquisition. The offeror must include their Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. The clauses are available in full text at http://www.acquisition.gov/far/. Interested vendors capable of furnishing the government with the products specified in this synopsis should submit a copy of their quotation to the below address or via email to [email protected]. Offers must also be accompanied by descriptive literature, delivery timeframe, warranties and/or other information that demonstrates that the offer meets all of the foregoing requirements. Quotations will be due ten calendar days from the publication date of this synopsis or by August 10, 2018 before 10:00am EST. via email or postal mail. The quotation must reference "Solicitation number" NICHD-18-104. Quotations sent by postal mail or other mailing services must be submitted to the following address: Eunice Kennedy Shriver National Institute of Child Health and Human Development 6710B Rockledge Drive, Room 1159B, MSC 7000 Bethesda, MD 20892. Note: Fed Ex/Ups/Ex/Ups/another courier use 20817. Attention: Tina Robinson, by the date and time mentioned above. Any questions must be sent via email to [email protected] and must include solicitation# NICHD-18-104 in the subject line of email. Faxed copies/responses will not be accepted.
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