The National Institute of Environmental Health Science (NIEHS) intends to award a Purchase Order to Activ Motif at 1914 Palomar Oaks Way, Carlsbad, CA 92008 for "ChIP" (for Chromatin ImmunoPrecipitation) followed by next generation Sequencing (ChIP-seq) or Polymerase Chain Reaction (ChIP-PCR) of frozen uterine tissue from mice for the Reproductive & Developmental Biology Laboratory at NIEHS.

The North American Industry Classification System (NAICS) Code is 541714 with a size standard of 1,000 employees. THIS IS A NOTICE OF INTENT TO AWARD AS A SOLE SOURCE, NO SOLICITATION OR REQUEST FOR QUOTE (RFQ) IS AVAILABLE. This notice of intent is NOT a request for competitive proposals. It has been determined that Activ Motif is the only known responsible source capable of providing the required service and no other service will satisfy the agency's requirements.

This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and in accordance with simplified acquisition procedures as stated in FAR Part 13.106-1(b)(1), and is exempt from the requirements of FAR Part 6.

The purpose of this acquisition is to:
1. Cross-link transcriptional proteins to chromatin within the tissue provided
2. Fragment and isolate the chromatin
3. Enrich the chromatin for estrogen receptor alpha (ERα), RNA polymerase II (PolII), p300 or cohesin using immunoprecipitation
4. Perform qPCR to detect co-precipitated DNA regions (ChIP-PCR) and provide us with the data OR construct libraries from the enriched chromatin for next-generation sequencing
5. Sequence all the chromatin DNA in the immunopreciptated/enriched chromatin using Next-generation sequencing
6. Provide data detailing all the sequences that interact with ERα, PolII, p300 or cohesin in each sample

This notice of intent is NOT a request for competitive proposals. However, the Government shall consider all responses received within 15 (fifteen) days of this posting, July 7, 2017, at 5pm EST. Interested parties may identify their interest and capability to respond to the requirement. A determination not to compete this requirement, based upon responses to this notice, is solely within the discretion of the Government. NIEHS will not be responsible for any costs incurred by interested parties in responding to this notice. If no affirmative responses are received by the date and time specified, an award will be made to Activ Motif, a small business.

Please email responses regarding this requirement to Julie Bardo: [email protected]