Sole Source FBO Notice for Procurement Request 000HCCCB-2014-72838
Title: Fracking: Toxicological Effect s of Silica and Diesel Exhaust Exposure
The Centers for Disease Control and Prevention (CDC) and the National Institute for Occupational Safety and Health (NIOSH), Toxicology and Molecular Biology Branch (TMBB) , Health Effects Laboratory Division (HELD) intends to award a sole source firm fixed price purchase order to West Virginia University Research Corporation, 866 Chestnut Ridge Road, Morgantown, WV 26506 to isolate RNA from rats exposed to silica and diesel exhaust particles.
Clin 1:
Quantity of (1) project - RNA Sequencing: TruSeq RNA library prep, quality control, secquencing run on HiSeq instrument.
STATEMENT OF WORK
Title of Project:
Fracking: Toxicological Effects of Silica and Diesel Exhaust Exposure
Background and Need
The services contemplated under this contract are considered to be an "Other Function" as defined by
the Office of Federal Procurement Policy in OFPP letter 11‐01 and by HHS in Acquisition Policy
Memorandum 2012‐01.
Illumina's next generation sequencing is a cutting edge technique and one of its applications is
the determination of global gene expression profile in biological samples. It is very well
understood that global gene expression profile in biological samples change in response to
exposure to toxic agents. Therefore, determination of global gene expression changes in response
to exposure to a toxic agent and further analysis of the gene expression data will result in the
identification of mechanisms potentially underlying the toxicity of the agents being studied. The
next generation sequencing service will help us to obtain global gene expression data required to
understand the molecular mechanisms underlying the toxicity of silica and diesel exhaust
particles. Our lab or any other lab at NIOSH, Morgantown, does not have the facility to do next
generation sequencing or any other technique that can be employed to determine global gene
expression profile in biological samples.
Project Objective
The project objective is to determine the molecular mechanisms underlying the toxicity due to exposure
to silica and diesel exhaust particles in a rat toxicity model.
Scope of Work
The Molecular Carcinogenesis Laboratory will isolate RNA from rats exposed to silica and diesel exhaust
particles. These RNA samples will then be sent to Genomics Sequencing Core, Biology Department,
Eberly College of Arts and Sciences, West Virginia University dba WVU Research Corporation to determine the gene expression profile
by next generation sequencing technique. The results will then be delivered electronically to the P.I. /
Molecular Carcinogenesis Laboratory for further analysis.
Technical Requirements
Genomics Sequencing Core, Biology Department, Eberly College of Arts and Sciences, West Virginia
University will supply all reagents, etc. and perform next generation sequencing using the next generation
sequencing system. The results will be delivered electronically to the P.I. / Molecular Carcinogenesis
Laboratory.
Reporting Schedule
The results will be delivered electronically to the P.I. / Molecular Carcinogenesis Laboratory upon
completion of the work.
Deliverables
The RNA samples will be personally delivered to the Genomics Sequencing Core, Biology Department,
Eberly College of Arts and Sciences, West Virginia University by the PI or another member of the
Molecular Carcinogenesis Laboratory. The Genomics Sequencing Core will perform next generation
sequencing and the resulting data will be processed. The raw and processed data will be delivered
electronically to the PI, Molecular Carcinogenesis Laboratory one year after the starting date of the
service.
This contract action is for a product for which the government intends to solicit and negotiate with only one source under the authority of FAR 13.106-1(b) and 10 U.S.C 2304(c) (1). Interested persons may identify their interest and capability to respond to the requirement or submit proposals.
The notice of intent is not a request for competitive quotations; however, all quotations/responses received within (15) days of the issuance of this notice will be considered by the Government. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. Any quotation/response should be e-mailed to purchasing agent Rebecca Mullenax at [email protected] by 11:59 p.m. Eastern time on June 11, 2014.
FAR Reference: FAR 5.207 - Preparation and transmittal of synopses under (c) (15), (c) (16) (i) and (c) (16) (ii).

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