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HIDEX Sense Multi-Technology Microplate Reader for Luminescence (LUM), Fluorescence Intensity (Fl), Time Resolved Fluorescence (TRF), Fluorescence Polarization (FP) and Absorbance (ABS) measurements. Reader is robot accessible. Includes MS Windows based touch screen operated instrument control/data acquisition software and high performance optics for reading of micro-well plates up to 1536 wells.
Features:
Shaking prior to measurement of each plate or well
Scanning of wells - 1 OOum steps over entire plate area
Temperature Control from 2C above ambient to 65C
Measurement time/plate
- Depends on the plate and measurement type
- Minimum <14 seconds for 96 well plate
Light Source
- Xe-flash lamp for Fl, TRF, FP and Abs modes
- Excitation/wavelength range 230 nm - 1000 nm
- Continuous closed loop intensity control
Light Path
- High intensity direct illumination optics
- Filter storage for 32 15mm or 12. 7 mm fluorescence
filters
- Filter and dichroic mirror additions/changes through
automatic hatch on instrument front
Detectors
Single PMT for detection for Fluorescence and
Luminescence
- Photon Counting for reading luminescence emission
- Integral counting for Fl and FP
- Photon counting with Time Resolved Decay Curve on
TRF
- Spectral range from 230 nm to 850 nm (PMT)
Absorbance Mode
- Spectrograph detector
- Spectral range from 230nm to 1 OOOnm
Fluorescence Mode
- Filter storage for 32 Filters, 12.7 or 15 mm diameter
- Wavelength Range: 230-850 nm
- Kinetic and endpoint measurements.
- Detection limits (> 3 S.D. above blank)
- Dynamic range up to 6 decades.
- Fluorescence Intensity (Fl): < 0.15 fmol fluorescein/ well in a black 384-well plate, 50uL
sample vol.
- Time-resolved Fluorescence (TRF): < 3 amol Eu/well in a white 384-well plate. 50uL sample vol.
- Fluorescence Polarization (FP): < 5 (typical < 3 mP stdev) at 1 nM fluorescein, in a black 96
well plate, 200 uL sample vol.
Luminescence Mode
- Kinetic and endpoint measurements .
- Glow Luminescence with or without filters
- Dynamic range up to 6 decades
- Sensitivity < 10 pM glow luminescence in a white 96-well plate (> 3 S.D. above blank)
- Background < 200 CPS
- Cross-talk (sample volume 200 uL):
<0.01 % on white 96-well plate, sample vol. 200 ml. <0.5% on white 384-well plate, sample vol. 50
ml.
Absorbance Mode
Kinetic and endpoint measurements . Wavelength Range 230-1000 nm
Performance at 450nm using 200uL sample volume: Accuracy 1% at 20D
Repeatability 0.8% at 20D
Dynamic Range 4.0 OD on 96 well plate Optical Filters included:
Filter 330nm for TRF-Eu & TR-FRET Filter 620nm for TR-FRET Eu
Filter 616nm for TRF-Eu & TR-FRET Filter 665nm for TR-FRET
Filter 355nm for Umbelliferone Filter 485nm for Fluoro FITC Filter 544nm for Rhodamine
Filter 460nm for Umbelliferone & BRET Filter 535nm for Fluoro FITC & BRET Additional filters available upon request
The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), has a requirement to carry out studies of glucose metabolism on a cellular and systemic level in cell culture, tissues, and whole animal that require the use of fluorescent, luminescent, and absorbance assays in multi-well plates. As part of our overall studies on the interplay between Epo and glucose metabolism, the ability to quickly and accurately measure a variety of compounds is crucial in better understanding glucose metabolism. The system must be quantitative and automated to provide for consistent and reproducible results. A small footprint
and light weight for portability are also required due to space limitations and the need for mutli-researcher use.
The microplate reader system should be automated with a high performance multimode microplate reader, compatible with 1-1536 well plates featuring five detection modes with top and bottom read. The system should also include temperature control and microplate shaking. The system needs an optical filter system with the capacity for 32 optical filters for a wide variety of fluorescent and luminescent assays without manual filter replacement. Absorbance assays need to scanned over a large wavelength spectrum in a minimal amount of time. The
microplate reader needs to have an integrated computer interface that offers ease-of-use and comes with the option of mouse/keyboard and touchscreen capabilities. The system should have state-of-the-art technology to produce accurate and reliable results in a quick and efficient manner. All hardware and software components need to be of recent design using solid-state electronics and modern robotics.
The Hidex Sense Microplate Reader is a compact, application ready high performance
multimode microplate reader, compatible with 1 to 1536 well plates, featuring five detection modes with top and bottom read and automatic height adjustment. The system also includes temperature control (ambient + 4 °C to 65°C), which allows for a wider range of temperature experiments, and versatile microplate shaking that ensures consistency between plates, runs, and
experiments. This system updates our current system and provided greater flexibility to accommodate our many assays for cell analysis such as viability, calcium uptake, enzymatic activity, and enzyme-based quantification of specific proteins. An optical filter system with large on-board capacity (32 filters) enables direct readiness with optimal performance for a wide variety of fluorescent and luminescent assays, without manual interaction. Absorbance assays are
measured utilizing a spectrograph detector, providing complete (230nm0100nm)spectrum readout and in less than a second per sample. Several wavelengths can be measured simultaneously. This greatly expands the number of assays that can be carried out without manually changing the filters and increases the information output by providing a complete spectral scan, important in hemoglobin analysis.
The "equal" item must meet the characteristics of the brand name item to be acceptable for award. Basis for Award: Offerors must provide descriptive literature or other material that demonstrates their offer meets the forgoing requirements. The contract will be awarded to the responsible offeror whose offer fully meets the requirements of the solicitation, provides the best value, with price and technical factors equally important in award evaluation.
This request is for interested firms with the capability of providing the required equipment listed above are requested to submit a capability statement to assist the Government in determining in accordance with Federal Acquisition Regulation (FAR) 19.502-2(b) whether or not this procurement will be set-aside for any of the programs described above. The intended procurement will be classified under North American Industrial Classification (NAICS) code 334516 with a size standard of 500. All respondents are requested to identify their firm's size and type of business. Interested firms responding to this market survey must provide (a) capability statement demonstrating their experience, skills and capability to fulfill the Government's requirements for the above. The capability statement shall be in sufficient enough detail, but not to exceed 15 pages, so that the Government can determine the experience and capability of your firm to provide the requirements above. Your capability statement, not to exceed 15 pages, should include references. Capability statement must be received at the address identified in this synopsis no later than 11:00 A.M. local time at designated location) on August 17, 2015.
This synopsis is for information and planning purposes and is not to be construed as a commitment by the Government, nor will the Government pay for information solicited.
Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provided feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate response to a solicitation. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
Any questions regarding this announcement must be sent via email to [email protected] and must include solicitation NIHLM2016180 in the subject line of email.
PLEASE NOTE: In order to receive an award, contractor must be registered and have valid certification in the System for Award Management (SAM) [formerly CCR].