Question Received :

I will need to know the exact requirements for excitation and emission for both sets of filters.

Example: Excitation + Emission #1 need to be  EX 340+/- 30nm + EM 450 +/-25nm
                    Excitation + Emission #2 need to be EX 300-370nm + EM 400-475nm

Your RFQ lists only approximate ranges for both filter sets.  Can you provide clarification?

Reply: 

An instrument that meets the EPA's minimum requirements shall have filter combinations to measure fluorescence at 2 different portions of a CDOM Excitation Emission Matrix. This means there should be no overlap of emission wavelengths, including filter bandpasses.

An example :

emission filter 1 = 420 nm +/- 20 nm bandpass

emission filter 2 = 470 nm +/- 20 nm bandpass

The excitation filters shall be selected between Exc = 300-370 nm, where, either one or two different wavelengths could be chosen. Two combination examples could be:

(1) Ex/Em 1 = 350 / 420 nm; Ex/Em 2 = 370 / 470 nm

(2) Ex/Em 1 = 350 / 420 nm; Ex/Em 2 = 350 / 470 mn

The choice of filters, in conjunction with lamp and detector design of a specific manufacturer should be based on optimizing CDOM fluorescence detection in natural waters.

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