PERFORMANCE WORK STATEMENT

The Virology & Emerging Infections Department of the U.S. Naval Medical and Research Unit No. 6 (NAMRU-6) is seeking to purchase primers and probes to be used for molecular diagnosis of Dengue virus (four serotypes), Chikungunya virus, Zika virus, Orthobunyavirus (including Guaroa, Oropouche, Group C, Bunyamwera and California viruses), Influenza virus (Influenza A-matrix gene and B) and its subtypes (H3, H1pdm/SwH1N1, H1 seasonal). Primers and probes will be used for reverse transcription (RT) real-time PCR assays using serum samples from febrile surveillance protocol and pharyngeal swabs sample from respiratory surveillance protocol.

All these NAMRU-6 assays have been validated using the TaqMan 7500/7500 Fast Real time PCR system and Quant studio platforms. The RT real-time PCR assays have been developed independently for each SOP and involve much time and effort by laboratory technicians, and supervisors for final approval. Reagents used must remain the same as the ones indicated in the SOPs, otherwise the design and validation of these processes would have to be initiated from zero, which would delay all processing and the timely diagnosis of public health relevant pathogens.

Materials should be shipped to the following address:

American Embassy - NAMRU-6

Attn: Virology & Emerging Infections Department

3230 Lima Place

Washington DC 20521