See Attached RFQ for Details and Terms

The Contractor shall provide the following:

1. Single-stranded DNA oligonucleotides:
   1. Length is understood  to be a significant difference in the construction, processing, and pricing of oligos:
   2. For single-stranded DNA oligonucleotides of up to 60 nucleotides (nt) in length:
      1. The turnaround time (TAT) will be one (1) business day with an ordering cutoff of 10:00 a.m. EST (to be shipped same-day overnight for next-day delivery).
      2. The expected minimum yield will be 25 nMole resuspended at 100 μM in nuclease-free water in a 96-well plate format, with ordering volumes of up to 8 plates per day.
   3. For single-stranded DNA oligonucleotides of up to 80 nucleotides (nt) in length:
      1. The turnaround time (TAT) will be one (1) business day with an ordering cutoff of 9:00 a.m. EST (to be shipped same-day overnight for next-day delivery).
      2. The expected minimum yield will be 25 nMole resuspended in tubes at 100 μM in nuclease-free water.
2. DNA/gene fragments:
   1. As with oligos, length is considered a significant factor in the manufacturing of gene fragments, and thus this task can be understood as having different expectations based on length.
   2. Double-stranded DNA gene fragments of 300-1,500bp (base pairs) in length with an error rate of less than 1 bp in 5,000bp. The turnaround time (TAT) will be three (3) business days with an ordering cutoff of 11:00 a.m. EST (with the ordering date being day 0). The expected minimum yield will be 200ng delivered resuspended at 10 ng/µl in a minimum of 20 µl of nuclease-free water.
   3. Double-stranded DNA gene fragments of 1,501-2,000bp (base pairs) in length with an error rate of less than 1 bp in 5,000bp. The turnaround time (TAT) will be eight (8) business days with an expected minimum yield of 1,000ng delivered lyophilized in tubes (with the ordering date being day 0).
   4. Double-stranded DNA gene fragments of 2,001-2,250bp (base pairs) in length with an error rate of less than 1 bp in 5,000bp. The turnaround time (TAT) will be eight (8) business days with an expected minimum yield of 1,000ng delivered lyophilized in tubes (with the ordering date being day 0).