The National Institutes of Health (NIH) National Institute Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to procure targated knock-in of a gene in the mouse genome. The goal of the work is to generate a mouse strain that carries a specific, heritable alteration in one of its genes. The task involves the application of gene targeting technology which is based on the process of homologous recombination to introduce the mutation into the desired gene. This is performed in mouse embryonic stem cells which are used to create founder mice carrying the targeted mutation. The mutation should be transmitted through the germline of the founder mice to allow generation of a colony of mice carrying the mutation for analysis. Specific requirements are: 1) Expertise in the field of gene targeting technology and a proven record of accomplishment. The provider should have been successful for a significant number of years in this field. Evidence for proven success includes the publication of a significant number of successful projects in reputable, peer-reviewed scientific journals. 2) Proven experience in performing similar projects in embryonic stem cell lines of different ,mouse strain origin. This includes the option of using C57BL/6- or 129 substrain-derived ES cells, each with high probabilities of success, supported by evidence from publications in peer¬reviewed scientific journals. 3) Ability to perform the tasks within a realistic, competitive time frame for an important scientific project. The statement of work will provide more information. All responsible businesses that are capable of providing the Government service listed above may request a copy of the Request for Quote (RFQ) in writing or by FAX to 301-480-8501 to the Contracting Office prior to the closing date of this notice. It is anticipated that RFQ NIH-NIDDK-07-226 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is May 7 2007. Request for copies of the RFQ must be submitted to the National Institute of Diabetes and Digestive and Kidney Diseases, 6707 Democracy Boulevard, Two Democracy Plaza, Room 774B, Bethesda, MD 20817 or via email: [email protected] to the Attention: V. Lynn Griffin. FAX quotations must contain offerors name, address, telephone number and fax number. FAX quotations will be accepted if dated and signed by an authorized company representative. All requests for quotations must have a valid email address.

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