The USDA, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska, intends to enter into a single source contract with Roche Diagnostics, Indianapolis, Indiana, for reagents. The reagents to be purchased are:

05 233 526 001
GS Titanium Sequencing Kit XLR70 (200 cycle - 70 x 75) For use with the GS Titanium PicoTiterPlate Kit 70x75

05 233 682 001
GS Titanium PicoTiterPlate Kit 70x75 - PTP with unique metallic coating to be used only with Titanium emPCR and Titanium Sequencing Kit XLR70.  Plate uses all of the same gaskets that are currently available (2-, 4, 8- and 16-region).

05 618 428 001
GS Titanium emPCR Kit (Lib-L) - emPCR kit V2 for the 2-region 70x75 PTP; Polymerase included

05 233 658 001
GS Titanium emPCR Breaking Kits LV/MV 12pc - Kit contains 12 individually packaged breaking devices

05 608 228 001
GS Rapid Library Prep Kit - For preparation of 12 shotgun type ligated libraries

05 463 343 001
Titanium Paired End Adapter Kit - For preparation of 10 paired-end libraries

Roche is the sole supplier an distributor of the GS FLX instrument.  The GS titanium library preparation reagents, emulsion based PCR kits, sequencing kits, and PicoTiter Plate Kits are only available through Roche.  The GS FLX warranty is conditional that customers agree to use the instrument in strict accordance with Roche operating instructions.  Other interested parties may submit questions and/or qualifications statements by Close of Business on May 22, 2010.