This acquisition shall consist of the following items:
Line 1 - ARRAY, DROGENE-1.0-ST, 6PK EACH, SKU#: 902155. Quantity: 2.
Line 2 - GENECHIP WT PLUS REAGENT KIT 30 RXN, SKU#: 902281. Quantity: 2.
Line 3 - KIT, DROGENE-1.0-ST ARRAY 30PK, SKU#: 902156. Quantity: 3.

(vi) The National Institute of Neurological Disorders and Stroke (NINDS) requires a set of oligonucleotide microarrays that allow accurate measurement of the expression levels of all the exons of all the genes in the Drosophilia genome. This is known as “expression profiling”. A large number of these arrays are required to measure expression level in both normal and mutant animals, at multiple ages, separately for the head and the body, and to do enough repeats for appropriate statistical analysis. Use of these chips will allow the NINDS to separate how a neurodegenerative mutation accelerates aging from how it causes death. NINDS participates in a trans-NIH Institute & Center shared resource for microarray experiments. For analysis of gene expression in Drosophila, the hardware, software and technical expertise available in the Microarray Facility are only compatible with the microarrays fabricated by the company Affymetrix. The salient characteristics for this requirement described in Attachment 1 – Purchase Description and shall include:
- The Affymetrix Drosophila GeneChip 1.0 ST can be used to assay expression of all the genes in the Drosophila genome.
- This chip is based on a recent annotation of the Drosophila genome, which corrects
previous errors and includes genes that were not identified previously.
- The 1.0 ST GeneChip uses a proprietary technology that allows assay of the entirety of each gene, rather than being weighted toward gene 3’ ends. This gives greater accuracy, and also allows separate detection and quantification of multiple splice variants rather than binning them together in uncontrolled ways that lose information and increase experimental noise.
- Use of this GeneChip allows accurate statistical comparison of our data to our previous results, and to results in the published literature.