The USDA, ARS, SEA has a requirement for a Saphyr System with Bionano Access Server, Saphyr Compute Server, and Saphyr Secondary Compute Server. The instrument includes a high-throughput nanofluidics system designed to generate physical genome maps from the massively parallel single-molecule visualization of extremely long DNA molecules without amplification to provide long-range contiguity critical for de novo sequence scaffolding and analysis of structural variation in complex genomes. The DNA molecules are differentially stained with fluorescent dyes, linearized in nanochannels, and imaged. The images are processed through the computing servers to assemble genome-spanning de novo physical maps. The Saphyr Chip is a proprietary, integrated nanofluidic chip comprised of 2 discreet flowcells each comprised of 120,000 nanochannels. The patented design of the chip allows for simultaneous, high-throughput interrogation of thousands of molecules of labeled DNA that are electrophoretically moved through the various features of the chip that linearize the molecules before they are confined in the nanochannels and imaged. The Saphyr Chip requires as little as 80ng of labeled DNA per flowcell with a data throughput of 320Gb per run. A solicitation is not available for this requirement and the USDA-ARS intends to make an award to Bionano Genomics on a sole source basis. Interested firms are encouraged to present proposals to the attention of Huron Virden as listed in this notice for commercial products which can perform the same function as presented above.

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